Spectral studies on the interaction between Cu2+ and urease

Abstract

The interactions of Cu(2+) with urease were investigated by fluorescence, UV/vis, CD, synchronous fluorescence, and three-dimensional fluorescence spectra techniques. Cu(2+) effectively quenched the intrinsic fluorescence of urease via static quenching. The binding constant K(A), the binding site n and the thermodynamic parameters are obtained. The process of binding Cu(2+) to urease was a spontaneous molecular interaction procedure with electrostatic interaction. The conformation of urease was discussed by UV/vis, CD, synchronous and three-dimensional fluorescence techniques.