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Comment
. 2012 Jun 15;590(12):2825-6.
doi: 10.1113/jphysiol.2012.233445.

Intestinal incretin responses to increased GLUT2 expression--Chacun à son goût

Richard J Naftalin  1
Affiliations
Comment

Intestinal incretin responses to increased GLUT2 expression--Chacun à son goût

Richard J Naftalin. J Physiol. .
No abstract available

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Figures

Figure 1
Figure 1. Panels abstracted from Mace et al. (2012). Panel A corresponds with Fig. 2B; panel B with Fig. 2E; panel C with Fig. 3C; panel D with Fig. 2H; and panel E with Fig. 5D
A, rat small intestine was apically perfused with 100 mm glucose in Krebs–Henseleit buffer (KHB) for 30 min in the presence of 0.5 mm phloridzin to inhibit SGLT1-mediated depolarisation of the membrane. Phloretin (0.5 mm; A) or cytochalasin B (10 μm; B) was used to inhibit GLUT2 activity. Serosal secretion samples were analysed for active GLP-1. C, apically perfused with 5 mm glucose containing 1 mm sucralose in KHB for 30 min. At t = 30 min, the perfusate was switched to an identical one without sucralose to determine whether stimulation was reversible. D, Na+ was replaced with choline to inhibit SGLT1- mediated depolarisation of the membrane. At t = 30 min, the perfusate was switched to an identical one containing phloretin. E, 5 mm glucose + phloridzin (0.5 mm) in KHB for 30 min. At t = 30 min, the perfusate was switched to an identical one containing either tolbutamide (500 μm) to block K+ATP-sensitive channels or diazoxide (340 μm) to open K+ATP-sensitive channels.
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