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. 2012 Jul;18(7):1204-6.
doi: 10.3201/eid1807.120385.

Culicoids as vectors of Schmallenberg virus

Culicoids as vectors of Schmallenberg virus

Lasse Dam Rasmussen et al. Emerg Infect Dis. 2012 Jul.
No abstract available

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Figures

Figure
Figure
RNA extracted from pools of Culicoides obsoletus group midges was tested in 1-step reverse transcription quantitative PCRs (RT-qPCRs) for the Schmallenberg virus large segment, and the products were analyzed by agarose gel electrophoresis. Lanes 1–8, C. obsoletus group midge pools 1–8; lanes 9–10; negative and positive controls, respectively. Numbers below lanes are cycle threshold values from RT-qPCRs; –, no value. M, size marker. Amplicons (145 bp) from positive pools were extracted and sequenced.
Figure A1
Figure A1
Location of trap site with culicoids positive for Schmallenberg virus (red dot), Hokkerup, Denmark, 2011.

References

    1. Hoffmann B, Scheuch M, Hoper D, Jungblut R, Holsteg M, Schirrmeier H, et al. Novel orthobunyavirus in cattle, Europe, 2011. Emerg Infect Dis. 2012;18:469–72. 10.3201/eid1803.111905 - DOI - PMC - PubMed
    1. Veronesi E, Mertens PP, Shaw AE, Brownlie J, Mellor PS, Carpenter ST. Quantifying bluetongue virus in adult Culicoides biting midges (Diptera: Ceratopogonidae). J Med Entomol. 2008;45:129–32. 10.1603/0022-2585(2008)45[129:QBVIAC]2.0.CO;2 - DOI - PubMed
    1. Toussaint JF, Sailleau C, Breard E, Zientara S, De Clercq K. Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments. J Virol Methods. 2007;140:115–23. 10.1016/j.jviromet.2006.11.007 - DOI - PubMed
    1. Hoffmann B, Bauer B, Bauer C, Batza HJ, Beer M, Clausen PH, et al. Monitoring of putative vectors of bluetongue virus serotype 8, Germany. Emerg Infect Dis. 2009;15:1481–4. 10.3201/eid1509.090562 - DOI - PMC - PubMed

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