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RNA extracted from pools of Culicoides obsoletus group midges was tested in 1-step reverse…
Figure
RNA extracted from pools of Culicoides obsoletus group midges was tested in 1-step reverse transcription quantitative PCRs (RT-qPCRs) for the Schmallenberg virus large segment, and the products were analyzed by agarose gel electrophoresis. Lanes 1–8, C. obsoletus group midge pools 1–8; lanes 9–10; negative and positive controls, respectively. Numbers below lanes are cycle threshold values from RT-qPCRs; –, no value. M, size marker. Amplicons (145 bp) from positive pools were extracted and sequenced.
Figure A1
Location of trap site with…
Figure A1
Location of trap site with culicoids positive for Schmallenberg virus (red dot), Hokkerup,…
Figure A1
Location of trap site with culicoids positive for Schmallenberg virus (red dot), Hokkerup, Denmark, 2011.
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