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. 2012 Jun;50(2):99-102.
doi: 10.3347/kjp.2012.50.2.99. Epub 2012 May 24.

IgG avidity ELISA test for diagnosis of acute toxoplasmosis in humans

Amir Hossien Rahbari  1 , Hossien Keshavarz, Saeedeh Shojaee, Mehdi Mohebali, Mostafa Rezaeian
Affiliations

IgG avidity ELISA test for diagnosis of acute toxoplasmosis in humans

Amir Hossien Rahbari et al. Korean J Parasitol. 2012 Jun.

Abstract

Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was performed by ELISA in duplicate rows of 96-well microtiter plates. One row was washed with 6 M urea and the other with PBS (pH 7.2), then the avidity index (AI) was calculated. Sixteen out of 18 (88.9%) sera with acute toxoplasmosis showed low avidity levels (AI ≤ 50), and 76 out of 82 (92.7%) sera in chronic phase of infection showed high avidity index (AI>60). Six sera had borderline ranges of AI. The results showed that the IgG avidity test by ELISA could distinguish the acute and chronic stages of toxoplasmosis in humans.

Keywords: ELISA; IgG avidity test; IgM; Toxoplasma gondii; indirect immunofluorescent antibody test.

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Figures

Fig. 1
Fig. 1
Values of avidity index (AI; %) in acute and chronic toxoplasmosis patients.
See this image and copyright information in PMC

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