doi: 10.1016/j.carres.2012.05.007.
Epub 2012 May 15.
One-pot four-enzyme synthesis of ketoses with fructose 1,6-bisphosphate aldolases from Staphylococcus carnosus and rabbit muscle
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- PMID: 22727596
- PMCID: PMC3396761
- DOI: 10.1016/j.carres.2012.05.007
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One-pot four-enzyme synthesis of ketoses with fructose 1,6-bisphosphate aldolases from Staphylococcus carnosus and rabbit muscle
Carbohydr Res.
.
Abstract
By the action of D-fructose 1,6-bisphosphate aldolases (FruA) from rabbit muscle and Staphylococcus carnosus, various ketoses were synthesized from glyceraldehydes or other aliphatic aldehydes as acceptors in a one-pot four-enzyme system.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Figures
SDS-PAGE analysis of FruAS.car expression and purification. Lanes: 1, whole cells not induced; 2, whole cells induced for 20 h; 3, cell lysate; 4, purified FruAS.car; 5, protein marker.
One-pot four-enzyme synthesis of ketoses with FruA (FruAS.car and RAMA).
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