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. 2012 Aug 1:357:143-6.
doi: 10.1016/j.carres.2012.05.007. Epub 2012 May 15.

One-pot four-enzyme synthesis of ketoses with fructose 1,6-bisphosphate aldolases from Staphylococcus carnosus and rabbit muscle

Zijie Li  1 , Li Cai, Mohui Wei, Peng George Wang
Affiliations

One-pot four-enzyme synthesis of ketoses with fructose 1,6-bisphosphate aldolases from Staphylococcus carnosus and rabbit muscle

Zijie Li et al. Carbohydr Res. .

Abstract

By the action of D-fructose 1,6-bisphosphate aldolases (FruA) from rabbit muscle and Staphylococcus carnosus, various ketoses were synthesized from glyceraldehydes or other aliphatic aldehydes as acceptors in a one-pot four-enzyme system.

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Figures

Figure 1
Figure 1
SDS-PAGE analysis of FruAS.car expression and purification. Lanes: 1, whole cells not induced; 2, whole cells induced for 20 h; 3, cell lysate; 4, purified FruAS.car; 5, protein marker.
Scheme 1
Scheme 1
One-pot four-enzyme synthesis of ketoses with FruA (FruAS.car and RAMA).
See this image and copyright information in PMC

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