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. 2012 Sep;74(1):88-90.
doi: 10.1016/j.diagmicrobio.2012.05.021. Epub 2012 Jun 22.

Spectrophotometry-based detection of carbapenemase producers among Enterobacteriaceae

Sandrine Bernabeu  1 , Laurent Poirel, Patrice Nordmann
Affiliations

Spectrophotometry-based detection of carbapenemase producers among Enterobacteriaceae

Sandrine Bernabeu et al. Diagn Microbiol Infect Dis. 2012 Sep.

Abstract

Carbapenem-hydrolyzing ß-lactamases are the most powerful ß-lactamases being able to hydrolyse almost all ß-lactams. They are mostly of the KPC, VIM, IMP, NDM, and OXA-48 type. A spectrophotometry technique based on analysis of the imipenem hydrolysis has been developed that differentiated carbapenemase- from noncarbapenemase producers. This inexpensive technique adapted to screening of carbapenemase producers may be implemented in any reference laboratory worldwide.

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