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. 2012 Jun;25(3):218-24.
doi: 10.2478/S13382-012-0027-5. Epub 2012 Jun 22.

Assessment of DNA damage using chromosomal aberrations assay in lymphocytes of waterpipe smokers

Enas S Alsatari  1 , Mohammad Azab, Omar F Khabour, Karem H Alzoubi, May F Sadiq
Affiliations

Assessment of DNA damage using chromosomal aberrations assay in lymphocytes of waterpipe smokers

Enas S Alsatari et al. Int J Occup Med Environ Health. 2012 Jun.

Abstract

Objectives: The aim of this study was to investigate the genotoxicity of waterpipe smoking in the lymphocytes of waterpipe smokers using chromosomal aberrations (CAs) assay.

Materials and methods: Fifty waterpipe smokers and 18 healthy non-smokers volunteered to participate in the study. Additionally, 18 heavy cigarette smokers were recruited for comparison. Chromosomal aberrations (CAs) assay was used to evaluate DNA damage in the lymphocytes.

Results: The results showed that similarly to cigarette smoking, waterpipe smoking significantly increased the frequencies of CAs (p < 0.01). In addition, the frequencies of CAs increased with more waterpipe use.

Conclusions: Waterpipe smoking causes DNA damage to lymphocytes and the damage increases with more waterpipe use.

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Figures

Fig. 1
Fig. 1
Levels of chromosomal aberrations in lymphocytes from smokers and healthy controls
Fig. 2
Fig. 2
Genotoxicity of waterpipe smoking increases with more waterpipe use
See this image and copyright information in PMC

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