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. 2012 Jul 11;12(7):3846-50.
doi: 10.1021/nl301857p. Epub 2012 Jun 29.

Atomic force microscopy with nanoscale cantilevers resolves different structural conformations of the DNA double helix

Carl Leung  1 , Aizhan Bestembayeva, Richard Thorogate, Jake Stinson, Alice Pyne, Christian Marcovich, Jinling Yang, Ute Drechsler, Michel Despont, Tilo Jankowski, Martin Tschöpe, Bart W Hoogenboom
Affiliations

Atomic force microscopy with nanoscale cantilevers resolves different structural conformations of the DNA double helix

Carl Leung et al. Nano Lett. .

Abstract

Structural variability and flexibility are crucial factors for biomolecular function. Here we have reduced the invasiness and enhanced the spatial resolution of atomic force microscopy (AFM) to visualize, for the first time, different structural conformations of the two polynucleotide strands in the DNA double helix, for single molecules under near-physiological conditions. This is achieved by identifying and tracking the anomalous resonance behavior of nanoscale AFM cantilevers in the immediate vicinity of the sample.

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Comment in

  • Upgrades for the AFM.
    Evanko D. Evanko D. Nat Methods. 2012 Aug;9(8):778-9. doi: 10.1038/nmeth.2117. Nat Methods. 2012. PMID: 23019689 No abstract available.

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