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Review
. 2012 Oct;60(10):723-33.
doi: 10.1369/0022155412453052. Epub 2012 Jun 27.

Imaging flow cytometry: coping with heterogeneity in biological systems

Natasha S Barteneva  1 , Elizaveta Fasler-Kan, Ivan A Vorobjev
Affiliations
Review

Imaging flow cytometry: coping with heterogeneity in biological systems

Natasha S Barteneva et al. J Histochem Cytochem. 2012 Oct.

Abstract

Imaging flow cytometry (IFC) platforms combine features of flow cytometry and fluorescent microscopy with advances in data-processing algorithms. IFC allows multiparametric fluorescent and morphological analysis of thousands of cellular events and has the unique capability of identifying collected events by their real images. IFC allows the analysis of heterogeneous cell populations, where one of the cellular components has low expression (<0.03%) and can be described by Poisson distribution. With the help of IFC, one can address a critical question of statistical analysis of subcellular distribution of proteins in a cell. Here the authors review advantages of IFC in comparison with more traditional technologies, such as Western blotting and flow cytometry (FC), as well as new high-throughput fluorescent microscopy (HTFM), and discuss further developments of this novel analytical technique.

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Conflict of interest statement

Declaration of Conflicting Interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Figure 1.
Figure 1.
A typical workflow for image flow cytometry.
See this image and copyright information in PMC

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