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. 2012 Apr 1;3(4):839-844.
doi: 10.3892/ol.2012.564. Epub 2012 Jan 12.

Immunohistochemical study of NF-κB p65, c-IAP2 and caspase-3 expression in cervical cancer

Li Xia  1 , Xiu-Zhen Xue
Affiliations

Immunohistochemical study of NF-κB p65, c-IAP2 and caspase-3 expression in cervical cancer

Li Xia et al. Oncol Lett. .

Abstract

The present study aimed to evaluate the expression of NF-κB p65, c-IAP2 and caspase-3 in human cervical cancer. Immunohistochemistry was performed to determine the levels of NF-κB p65, c-IAP2 and caspase-3 expression in cervical cancer. Our study found overexpression of NF-κB p65 and c-IAP2 and reduced expression of caspase-3 in cervical cancer and cervical intraepithelial neoplasia (CIN) II-III. The expression of these molecules had no significant differences in the various clinical stages of cervical cancer, but the expression levels of NF-κB p65 and c-IAP2 in poorly differentiated tumors and metastatic lymph nodes were higher than levels in well-differentiated tumors and non-metastatic lymph nodes. By contrast, caspase-3 expression in well-differentiated tumors and non-metastatic lymph nodes was higher than that in poorly differentiated tumors and metastatic lymph nodes. Overexpression of NF-κB p65 and c-IAP2 and reduced expression of caspase-3 in cervical cancer may be important in the tumorigenesis and development of cervical cancer, and they may be useful as biomarkers to estimate progression of cervical cancer.

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Figures

Figure 1
Figure 1
Immunohistochemical staining for expression of NF-κB p65, c-IAP2 and caspase-3. Expression of (A-D) NF-κB p65, (E-H) c-IAP2, (I-L) caspase-3 in normal cervix, CIN I, CIN III and cervical cancer, respectively. (M-P) Negative staining control. Each figure has original magnification, x100, with the exception of J, H and M. CIN, cervical intraepithelial neoplasia.
Table I
Table I
Expression of NF-κB p65, c-IAP2 and caspase-3 in cervical cancer, CIN and normal cervical tissue.
Table II
Table II
Expression of NF-κB p65, c-IAP2 and caspase-3 in different grades of CIN.
Table III
Table III
Expression of NF-κB p65, c-IAP2 and caspase-3 in different clinical stages and histological grades of cervical cancer as well as in metastatic lymph nodes.
Table IV
Table IV
Correlation analysis of NF-κB p65 and c-IAP2 expression in cervical cancer.
Table V
Table V
Correlation analysis of expression of NF-κB p65, c-IAP2 and caspase-3 in cervical cancer.
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