[A new method for determination of ATPase activity in tissue homogenates]
[Article in
Italian]
- PMID: 227435
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[A new method for determination of ATPase activity in tissue homogenates]
[Article in
Italian]
Boll Soc Ital Biol Sper.
.
Abstract
Authors describe a new method to determine Mg2+ and Na+K+ATPase activity on extremely small samples of tissue homogenates. They use specific inhibitors to discern between the two activities and a sensitive colorimetric method to dose Pi released from ATP, Lower limit of sensitivity is about 0,0100 micromoles of Pi/mg proteins/hour.
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