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. 1978 Dec 30;54(24):2577-82.

[A new method for determination of ATPase activity in tissue homogenates]

[Article in Italian]
G Martino, M Testa, P Vicinanza, E Habetswallner
  • PMID: 227435

[A new method for determination of ATPase activity in tissue homogenates]

[Article in Italian]
G Martino et al. Boll Soc Ital Biol Sper. .

Abstract

Authors describe a new method to determine Mg2+ and Na+K+ATPase activity on extremely small samples of tissue homogenates. They use specific inhibitors to discern between the two activities and a sensitive colorimetric method to dose Pi released from ATP, Lower limit of sensitivity is about 0,0100 micromoles of Pi/mg proteins/hour.

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