A need for speed: genetic encoding of rapid cycloaddition chemistries for protein labelling in living cells
- PMID: 22753121
- DOI: 10.1002/cbic.201200321
A need for speed: genetic encoding of rapid cycloaddition chemistries for protein labelling in living cells
Abstract
Rapid reactions: Several reactants for strain-promoted cycloaddition reactions have been genetically encoded as the side chains of noncanonical amino acids. This results in decisive improvements for the fluorescent labelling of intracellular proteins such as quantitative turnover, completion of labelling reactions within minutes, fluorogenic effects and even partial orthogonality for multicolour labelling.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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