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. 2012 Jan;76(1):1-7.

Plasma protein profiles of neonatal pigs before and after suckling

Yanyun Huang  1 , Douglas J Olson, John R Gordon, Dorothy M Middleton, Elemir Simko
Affiliations

Plasma protein profiles of neonatal pigs before and after suckling

Yanyun Huang et al. Can J Vet Res. 2012 Jan.

Abstract
in English, French

Absorption of colostral proteins ingested by neonatal piglets within 24 to 36 h after birth is generally considered to be non-selective. Nevertheless, the transfer of colostral proteins, except immunoglubulins, from gut to bloodstream after natural suckling is still poorly characterized. The purpose of this study was to investigate the changes in 2-dimensional electrophoretic plasma protein profiles of neonatal piglets before and after suckling, in order to characterize the gastrointestinal absorption of colostral proteins into the neonatal bloodstream. As expected, the most significant change in plasma after suckling is the presence of a large amount of immunoglobulin. However, while the concentration of a few proteins was mildly increased in post-suckling plasma, the evidence of absorption of colostral non-immunoglobulin proteins by neonatal piglets was not detected in this study.

L’absorption des protéines du colostrum ingérées par les porcelets nouveau-nés dans les 24 à 36 h suivant la naissance est généralement considérée comme étant non-sélective. Néanmoins, le transfert après la tétée des protéines du colostrum, à l’exception des immunoglobulines, de l’intestin vers le sang est pauvrement caractérisé. L’objectif de ce travail était d’étudier les changements des profils plasmatiques des protéines par électrophorèse en deux dimensions chez des porcelets nouveau-nés avant et après la tétée, afin de caractériser l’absorption gastro-intestinale des protéines du colostrum dans la circulation sanguine des nouveau-nés. Tel qu’attendu, le changement le plus significatif après la tétée est la présence d’une grande quantité d’immunoglobulines. Toutefois, alors que la concentration de quelques protéines était légèrement augmentée dans les échantillons de plasma post-tétée, aucune évidence d’absorption de protéines du colostrum autres que les immunoglobulines par les porcelets nouveau-nés ne fut détectée dans cette étude.

(Traduit par Docteur Serge Messier)

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Figures

Figure 1
Figure 1
Colloidal Coomassie G-250 stained 2-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D SDS-PAGE) of the proteins (80 μg) removed from 24 h post-suckling porcine plasma by protein-A affinity chromatography. By visual estimation, spots represent immunoglobulin heavy chain (~ 50 kDa) and light chain (~ 28 to 31 kDa) compose more than 95% of the total proteins in the elutant.
Figure 2
Figure 2
Representative images of Coomassie-stained 2-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D SDS-PAGE) of whole pre-suckling (A) and 24 h post-suckling (B) plasma. Immunoglobulins are present in large amount after suckling. Two additional spots (spot a and b) are prominent after suckling.
Figure 3
Figure 3
Representative images of Coomassie-stained 2-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D SDS-PAGE) of immunoglobulin-removed pre-suckling (A) and 24 h post-suckling (B) porcine plasma. Major plasma proteins (squares) and proteins increased prominently after suckling (ovals) are noted. Protein identification numbers correspond to those in Table I.
See this image and copyright information in PMC

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