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. 2012 Jun 1;4(3):247-58.
doi: 10.3978/j.issn.2072-1439.2012.06.02.

Five-year update on the mouse model of orthotopic lung transplantation: Scientific uses, tricks of the trade, and tips for success

Xue Lin, Wenjun Li, Jiaming Lai, Mikio Okazaki, Seiichiro Sugimoto, Sumiharu Yamamoto, Xingan Wang, Andrew E Gelman, Daniel Kreisel, Alexander Sasha Krupnick

Five-year update on the mouse model of orthotopic lung transplantation: Scientific uses, tricks of the trade, and tips for success

Xue Lin et al. J Thorac Dis. .

Abstract

It has been 5 years since our team reported the first successful model of orthotopic single lung transplantation in the mouse. There has been great demand for this technique due to the obvious experimental advantages the mouse offers over other large and small animal models of lung transplantation. These include the availability of mouse-specific reagents as well as knockout and transgenic technology. Our laboratory has utilized this mouse model to study both immunological and non-immunological mechanisms of lung transplant physiology while others have focused on models of chronic rejection. It is surprising that despite our initial publication in 2007 only few other laboratories have published data using this model. This is likely due to the technical complexity of the surgical technique and perioperative complications, which can limit recipient survival. As two of the authors (XL and WL) have a combined experience of over 2500 left and right single lung transplants, this review will summarize their experience and delineate tips and tricks necessary for successful transplantation. We will also describe technical advances made since the original description of the model.

Keywords: Mouse model; evaluation; orthotopic lung transplantation; surgical complications.

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Figures

Figure 1
Figure 1
10-0 Nylon for suturing tiny hole of bronchus.
Figure 2
Figure 2
Animal Surgical Glue for sealing small air leak of graft lung.
Figure 3
Figure 3
Diaphragm window for treatment of type II pulmonary injury.
Figure 4
Figure 4
MRI images obtained 3 days after lung transplantation reveal ventilation of lung graft on three serial coronal images (top) and graft atelectasis in a different animal (bottom). As previously described (29) aerated lung looks black on MRI due to lack of signal intensity while a consolidated lung looks white due to the high intensity of this water-rich structure when atelectatic.
Figure 5
Figure 5
Adjustment of bronchus cuff size. From L to R, the size is 18#, 20# and 24#, which are used for bronchus, pulmonary vein, and pulmonary artery, respectively.
Figure 6
Figure 6
Occlude the hilum of native non-transplanted lung with clip.
Figure 7
Figure 7
Occlude the hilum of native non-transplanted lung with 6-0 silk.
Figure 8
Figure 8
The current method for BAL.
Figure 9
Figure 9
Rapid substitution of professional antigen presenting cells, such as dendritic cells, occurs after orthotopic lung transplantation.
See this image and copyright information in PMC

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References

    1. Okazaki M, Krupnick AS, Kornfeld CG, et al. A mouse model of orthotopic vascularized aerated lung transplantation. Am J Transplant. 2007;7:1672-1679 - PubMed
    1. Gelman AE, Okazaki M, Sugimoto S, et al. CCR2 regulates monocyte recruitment as well as CD4 T1 allorecognition after lung transplantation. Am J Transplant. 2010;10:1189-1199 - PMC - PubMed
    1. Kreisel D, Lai J, Richardson SB, et al. Polarized alloantigen presentation by airway epithelial cells contributes to direct CD8+ T cell activation in the airway. Am J Respir Cell Mol Biol. 2011;44:749-754 - PMC - PubMed
    1. Kreisel D, Richardson SB, Li W, et al. Cutting edge: MHC class II expression by pulmonary nonhematopoietic cells plays a critical role in controlling local inflammatory responses. J Immunol. 2010;185:3809-3813 - PMC - PubMed
    1. Krupnick AS, Lin X, Li W, et al. Orthotopic mouse lung transplantation as experimental methodology to study transplant and tumor biology. Nat Protoc. 2009;4:86-93 - PMC - PubMed