Human semen quality in the new millennium: a prospective cross-sectional population-based study of 4867 men

Abstract

Objectives: Considerable interest and controversy over a possible decline in semen quality during the 20th century raised concern that semen quality could have reached a critically low level where it might affect human reproduction. The authors therefore initiated a study to assess reproductive health in men from the general population and to monitor changes in semen quality over time.

Design: Cross-sectional study of men from the general Danish population. Inclusion criteria were place of residence in the Copenhagen area, and both the man and his mother being born and raised in Denmark. Men with severe or chronic diseases were not included.

Setting: Danish one-centre study.

Participants: 4867 men, median age 19 years, included from 1996 to 2010.

Outcome measures: Semen volume, sperm concentration, total sperm count, sperm motility and sperm morphology.

Results: Only 23% of participants had optimal sperm concentration and sperm morphology. Comparing with historic data of men attending a Copenhagen infertility clinic in the 1940s and men who recently became fathers, these two groups had significantly better semen quality than our study group from the general population. Over the 15 years, median sperm concentration increased from 43 to 48 million/ml (p=0.02) and total sperm count from 132 to 151 million (p=0.001). The median percentage of motile spermatozoa and abnormal spermatozoa were 68% and 93%, and did not change during the study period.

Conclusions: This large prospective study of semen quality among young men of the general population showed an increasing trend in sperm concentration and total sperm count. However, only one in four men had optimal semen quality. In addition, one in four will most likely face a prolonged waiting time to pregnancy if they in the future want to father a child and another 15% are at risk of the need of fertility treatment. Thus, reduced semen quality seems so frequent that it may impair the fertility rates and further increase the demand for assisted reproduction.

Figure 1

Semen parameters of Danish men from the general population. Red bars show 25th–75th percentiles with median line. Whiskers show 5th–95th percentiles. The sperm concentration (A) and total sperm count (B) increased slightly by year of examination. Percentage of morphologically normal spermatozoa did not show any temporal trend (C).

Figure 2

Distributions of sperm counts and morphologically normal spermatozoa in Danish men from the general population and fertile Danish men (partners of pregnant women). All men had durations of ejaculation abstinence above 48 h. Sperm concentration (A) total sperm counts (B) and percentages of morphologically normal spermatozoa (C) were lower in men from the general population.

Figure 3

Distributions of sperm counts in Danish men from the general population, examined from 1996 to 2010 and Danish men examined in an infertility clinic in the 1940s. All men had durations of ejaculation abstinence above 48 h. Sperm concentration (A) and total sperm counts (B).