Comparative Study
doi: 10.1055/s-0028-1092761.
Immunogenicity and bioactivity of glucagon, modified at methionine-27
- PMID: 227776
- DOI: 10.1055/s-0028-1092761
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Comparative Study
Immunogenicity and bioactivity of glucagon, modified at methionine-27
Horm Metab Res.
1979 Aug.
Abstract
Porcine glucagon was modified at methionine-27 by methylation or oxidation. Antisera against the glucagon derivatives were obtained. One of these antisera showed a high affinity for glucagon, with no cross-reactivity with gut-GLI 1. Biological activities of these derivatives were assessed on rat hepatocytes. Both derivatives had the same maximal glucose-mobilising activity as native glucagon, but a decrease potency, suggesting a crucial role of methionine in the binding of glucagon to its hepatic receptor.
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