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. 2012:2012:390107.
doi: 10.1155/2012/390107. Epub 2012 Jun 19.

cDNA-SRAP and its application in differential gene expression analysis: a case study in Erianthus arundinaceum

Youxiong Que  1 , Liping Xu, Jianwei Lin, Jun Luo, Jingsheng Xu, Jin Zheng, Rukai Chen
Affiliations

cDNA-SRAP and its application in differential gene expression analysis: a case study in Erianthus arundinaceum

Youxiong Que et al. J Biomed Biotechnol. 2012.

Abstract

Erianthus arundinaceum is a wild relative species of sugarcane. The aim of this research was to demonstrate the feasibility of cDNA-SRAP for differential gene expression and to explore the molecular mechanism of drought resistance in E. arundinaceum. cDNA-SRAP technique, for the first time, was applied in the analysis of differential gene expression in E. arundinaceum under drought stress. In total, eight differentially expressed genes with length of 185-427 bp were successfully isolated (GenBank Accession numbers: EU071770, EU071772, EU071774, EU071776, EU071777, EU071779, EU071780, and EU071781). Based on their homologies with genes in GenBank, these genes were assumed to encode ribonuclease III, vacuolar protein, ethylene insensitive protein, aerobactin biosynthesis protein, photosystem II protein, glucose transporter, leucine-rich repeat protein, and ammonia monooxygenase. Real-time PCR analysis on the expression profiling of gene (EU071774) encoding ethylene-insensitive protein and gene (EU071781) encoding ammonia monooxygenase revealed that the expression of these two genes was upregulated both by PEG and ABA treatments, suggesting that they may involve in the drought resistance of E. arundinaceum. This study constitutes the first report of genes activated in E. arundinaceum by drought stress and opens up the application of cDNA-SRAP in differential gene expression analysis in E. arundinaceum under certain stress conditions.

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Figures

Figure 1
Figure 1
The amplified result of eight primer combinations. Notes: M, Marker; T, Treatment; C, Control; B, Blank or negative control; P1–P8, primer combinations.
Figure 2
Figure 2
Validation of 10 differentially expressed genes screened in cDNA-SRAP by Real-time PCR.
Figure 3
Figure 3
Expression profiling of ethylene insensitive gene (EU071774) in E. arundinaceum under PEG (a) and ABA (b) treatments.
Figure 4
Figure 4
Expression profiling of ammonia monooxygenase gene (EU071781) in E. arundinaceum under PEG (a) and ABA (b) treatments.
See this image and copyright information in PMC

References

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