Endotoxin in vitro interactions with human neutrophils: depression of chemiluminescence, oxygen consumption, superoxide production, and killing
- PMID: 227788
- PMCID: PMC414534
- DOI: 10.1128/iai.25.3.912-921.1979
Endotoxin in vitro interactions with human neutrophils: depression of chemiluminescence, oxygen consumption, superoxide production, and killing
Abstract
Endotoxin was shown to depress neutrophil bactericidal activity while enhancing Nitro Blue Tetrazolium reduction and hexose monophosphate shunt activity. Separation of bactericidal action from oxidative metabolism suggests that the effect of endotoxin might involve the formation of reactive oxygen radicals such as superoxide. Chemiluminescence often accompanies metabolic activation of polymorphonuclear neutrophils (PMNs). However, human PMNs did not show chemiluminescence when challenged with endotoxin (lipopolysaccharide; LPS) or lipid A. Superoxide formation was also unaffected by endotoxin. In contrast, preincubation of PMNs with LPS for 30 min produced significant depression of chemiluminescence, oxygen consumption, and superoxide formation. Decreased chemiluminescence was not the result of complement consumption. In a cell-free system, superoxide was not scavenged by LPS, nor did LPS stimulate superoxide dismutase. Oxidase enzymes for reduced nicotinamide adenine dinucleotide or reduced nicotinamide adenine dinucleotide phosphate harvested from broken cells were not affected by LPS. The toxicity of LPS may reside in its ability to activate the PMNs while simultaneously blocking bactericidal capacity.
Similar articles
-
Chemiluminescence and the study of phagocyte redox metabolism.Adv Exp Med Biol. 1982;141:411-21. doi: 10.1007/978-1-4684-8088-7_39. Adv Exp Med Biol. 1982. PMID: 6283834 No abstract available.
-
The in vitro effect of sulbactam on polymorphonuclear leukocyte function.Diagn Microbiol Infect Dis. 1989 Jul-Aug;12(4 Suppl):147S-152S. doi: 10.1016/0732-8893(89)90127-2. Diagn Microbiol Infect Dis. 1989. PMID: 2556238
-
Functional and metabolic studies of polymorphonuclear leukocytes in the congenital Pelger-Huet anomaly.Blood. 1980 Mar;55(3):466-9. Blood. 1980. PMID: 6244014
-
The oxidative killing mechanisms of the neutrophil.Prog Clin Immunol. 1977;3:1-65. Prog Clin Immunol. 1977. PMID: 17138 Review. No abstract available.
-
Effects of bacterial endotoxins on neutrophil function.Rev Infect Dis. 1985 May-Jun;7(3):404-18. doi: 10.1093/clinids/7.3.404. Rev Infect Dis. 1985. PMID: 3895355 Review.
Cited by
-
Carrageenan stimulates reduction of nitroblue tetrazolium by human neutrophils without membrane depolarization, myeloperoxidase secretion, or increased oxygen consumption.Inflammation. 1986 Dec;10(4):425-34. doi: 10.1007/BF00915826. Inflammation. 1986. PMID: 3025093
-
Stimulus-specific effects of endotoxin on superoxide production by rabbit polymorphonuclear leukocytes.Yale J Biol Med. 1987 Sep-Oct;60(5):391-6. Yale J Biol Med. 1987. PMID: 2827396 Free PMC article.
-
Simple procedure for measuring neutrophil chemiluminescence.J Clin Microbiol. 1981 Feb;13(2):390-2. doi: 10.1128/jcm.13.2.390-392.1981. J Clin Microbiol. 1981. PMID: 7204552 Free PMC article.
-
The effect of Brucella abortus on hydrogen peroxide and nitric oxide production by bovine polymorphonuclear cells.Vet Res Commun. 2002 Feb;26(2):93-102. doi: 10.1023/a:1014039500378. Vet Res Commun. 2002. PMID: 11922486
-
Induction of human granulocyte chemiluminescence by bacterial lipopolysaccharides.Infect Immun. 1987 Mar;55(3):758-61. doi: 10.1128/iai.55.3.758-761.1987. Infect Immun. 1987. PMID: 3818095 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources