Comment on

• Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase.

  Geiser M, Cèbe R, Drewello D, Schmitz R. Geiser M, et al. Biotechniques. 2001 Jul;31(1):88-90, 92. doi: 10.2144/01311st05. Biotechniques. 2001. PMID: 11464525
• Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

  Bryksin AV, Matsumura I. Bryksin AV, et al. Biotechniques. 2010 Jun;48(6):463-5. doi: 10.2144/000113418. Biotechniques. 2010. PMID: 20569222 Free PMC article.