Influence of inactivated Sendai virus on early events in polyomavirus infection of permissive and nonpermissive host cells

Abstract

Quantitative radiochemical investigations have been performed regarding the influence of betapropiolactone-inactivated Sendai virus on the early events of interaction of polyomavirus with permissive and nonpermissive cells. These experiments have shown: (i) Sendai virus did not increase polyomavirus adsorption on permissive or nonpermissive cells; (ii) Sendai virus induced polyomavirus elution from permissive and nonpermissive cells during the first 40 min and 6 h postinfection, respectively; and (iii) Sendai virus promoted the penetration of polyomavirus into lysosomes only in nonpermissive cells. The maximal amounts of radioactively labeled virion DNA and viral coat proteins were found in lysosomes at 2--3 h postinfection.