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. 2012 May;3(5):1069-1072.
doi: 10.3892/ol.2012.596. Epub 2012 Feb 9.

Kaempferol-3-O-rhamnoside isolated from the leaves of Schima wallichii Korth. inhibits MCF-7 breast cancer cell proliferation through activation of the caspase cascade pathway

Ajeng Diantini  1 , Anas Subarnas, Keri Lestari, Eli Halimah, Yasmiwar Susilawati, Supriyatna, Euis Julaeha, Tri H Achmad, Eka W Suradji, Chiho Yamazaki, Kenji Kobayashi, Hiroshi Koyama, Rizky Abdulah
Affiliations

Kaempferol-3-O-rhamnoside isolated from the leaves of Schima wallichii Korth. inhibits MCF-7 breast cancer cell proliferation through activation of the caspase cascade pathway

Ajeng Diantini et al. Oncol Lett. 2012 May.

Abstract

Plants consumed by non-human primates represent potential drug sources for human disease management. In this study, we isolated kaempferol-3-O-rhamnoside as an active compound from the leaves of Schima wallichii Korth., a plant commonly consumed by non-human primates. Its anti-cancer activities, including its ability to induce apoptotic mechanisms, were investigated in MCF-7 breast cancer cells. Results showed that in MCF-7 cells, kaempferol-3-O-rhamnoside inhibits cell proliferation in a dose-dependent manner and promotes apoptosis via the activation of the caspase signaling cascade, which includes caspase-9, caspase-3 and PARP. Our results provide a basis for further exploration of kaempferol-3-O-rhamnoside as an active compound for potential anti-cancer therapeutics.

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Figures

Figure 1
Figure 1
IC50 concentration of Schima wallichii Korth. extract and fractions for suppression of MCF-7 proliferation. Ethyl acetate fraction of Schima wallichii Korth. shows the strongest potential in inhibiting MCF-7 proliferation.
Figure 2
Figure 2
Identification of the major compound within the ethyl acetate fraction of Schima wallichii. (A) LC-MS profile of the isolated compound. (B) Structure of kaempferol-3-O-rhamnoside.
Figure 3
Figure 3
Effect of the 24-h treatment of kaempferol-3-O-rhamnoside on cancer (MCF-7) and non-cancer (HC-04) cells.
Figure 4
Figure 4
Western blot analysis of apoptotic proteins that are upregulated in MCF-7 cells treated with kaempferol-3-O-rhamnoside for 24 h and collected at the indicated times; * is the cleaved form.
See this image and copyright information in PMC

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