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. 1979 Jun;9(6):731-3.
doi: 10.1128/jcm.9.6.731-733.1979.

Collagenase in equine cell culture preparation

Collagenase in equine cell culture preparation

G Lang. J Clin Microbiol. 1979 Jun.

Abstract

Equine kidney cells disaggregated by treatment with 0.01% collagenase were used in the preparation of primary monolayer cell cultures. The primary cells could be stored for long periods in liquid nitrogen and subsequently subcultivated. These techniques provided a long-term supply of equine kidney cells, free of apparent contamination, from the kidneys of a single fetus.

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