Collagenase in equine cell culture preparation
- PMID: 227924
- PMCID: PMC275389
- DOI: 10.1128/jcm.9.6.731-733.1979
Collagenase in equine cell culture preparation
Abstract
Equine kidney cells disaggregated by treatment with 0.01% collagenase were used in the preparation of primary monolayer cell cultures. The primary cells could be stored for long periods in liquid nitrogen and subsequently subcultivated. These techniques provided a long-term supply of equine kidney cells, free of apparent contamination, from the kidneys of a single fetus.
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