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Clinical Trial
. 2012 Oct;29(10):1083-9.
doi: 10.1007/s10815-012-9831-x. Epub 2012 Jul 14.

The relationship between pregnancy and oxidative stress markers on patients undergoing ovarian stimulations

Abdelmoneim Younis  1 , Cynthia Clower, Deanna Nelsen, William Butler, Andrew Carvalho, Eden Hok, Mahdi Garelnabi
Affiliations
Clinical Trial

The relationship between pregnancy and oxidative stress markers on patients undergoing ovarian stimulations

Abdelmoneim Younis et al. J Assist Reprod Genet. 2012 Oct.

Abstract

Purpose: We investigated the activities and relevance of a validated panel of antioxidant enzymes, cytokines, specific lipid peroxidation end products and six fatty acids by correlational analyses with peak E(2) levels and pregnancy outcome after ovarian stimulation for IVF or IUI.

Methods: Blood samples obtained from 15 patients undergoing ovarian stimulation with rFSH or hMG were divided into two groups. Group-1 was baseline blood collected on day-2-3 of women cycle. Group-2 is blood collected at the end of FSH/hMG injection. Serum was collected and stored in liquid nitrogen at -196 °C until analysis. Standard IVF and IUI procedures were followed. The serum levels of Paraoxonase (PON1), Superoxide Dismutases (SOD), Interleukin-6 (IL-6), Glutathione Peroxidase (GPx), 8-Isoprostane, and fatty acids Arachidic, Palmitic, Stearic, Oleic, Linoleic & Linolenic were measured.

Results: With the exception of 8-Isoprostane, results showed a positive correlation between baseline and peak levels of E(2) and that of SOD, GPx, PON1, and IL-6. The PON1, IL-6 and SOD were significantly (p < 0.05) higher in pregnant than non-pregnant group. Fatty acid levels at baseline and peak E(2) were not different but pregnancy rates were found to be decreasing with higher palmitic, and stearic acid levels.

Conclusions: Ovarian stimulation causes a significant increase in serum PON1, SOD, GPx and IL-6 activity in women undergoing IVF or IUI. The high levels of IL-6, SOD, and PON1 and lower levels of palmitic, and stearic acids in the pregnancy positive group indicate that these oxidative stress and nutritional factors may be used as a predictive marker in controlled ovarian stimulation success.

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Figures

Fig. 1
Fig. 1
The levels of serum antioxidant enzymes, cytokines and oxidative stress markers collected at baseline prior to hormonal stimulation and at peak E2 on the day of hCG injection. PON = Paraoxonase, SOD = Superoxide Dismutases, IL-6 = Interleukin-6, and GPx = Glutathione Peroxidase. Mean values are shown on top of each data point
Fig. 2
Fig. 2
The levels of serum fatty acids at baseline prior to ovarian hormonal stimulation and at peak E2 on the day of hCG injection. Mean values are shown on top of each data point
Fig. 3
Fig. 3
Difference between pregnant and non-pregnant patients on the levels of serum fatty acids at baseline prior to ovarian hormonal stimulation. Mean values are shown on top of each data point
Fig. 4
Fig. 4
Difference between pregnant and non-pregnant patients on the levels of serum fatty acids at peak E2 level after ovarian hormonal stimulation. Mean values are shown on top of each data point
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