Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Oct 15;50(2):177-85.
doi: 10.1007/BF01868947.

Uncapping of viral messenger RNA by phosphodiesterase of fibroblast plasma membranes

S Harshman, J G Conlin, D Stoller, D L Harshman

Uncapping of viral messenger RNA by phosphodiesterase of fibroblast plasma membranes

S Harshman et al. J Membr Biol. .

Abstract

Isolated plasma membranes from mouse fibroblast lines 3T3 and its tranformant SV-3T3 contain a phosphodiesterase (oligonucleotidase, E.C. 3.1.4.19; nucleotide pyrophosphatase, E.C. 3.6.1.9) that splits capped and methylated messenger RNA obtained from both reovirus and vesicular stomatitis virus. The isolated membranes are free of demonstrable ribonuclease activity and split the mRNA to produce 7-methyl guanosine diphosphate as a product. With ATP as substrate for the phosphodiesterase enzyme, the product is AMP. Synthetic caps, AMP, ADP and ATP, but not cyclic AMP, can compete with the substrate p-nitrophenyl thymidilic acid. A possible regulatory role on messenger translation is proposed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Cell. 1975 Oct;6(2):185-95 - PubMed
    1. Anal Biochem. 1978 Oct 1;90(1):98-106 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2734-8 - PubMed
    1. Nature. 1975 May 1;255(5503):33-7 - PubMed
    1. Biochemistry. 1978 Aug 22;17(17):3627-32 - PubMed

Publication types

LinkOut - more resources