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. 2011;5(1):1-6.
Epub 2011 Jun 30.

PCR-Detection of Coxiella burnetii in Ticks Collected from Sheep and Goats in Southeast Iran

Sr Nourollahi Fard  1 , M Khalili
Affiliations

PCR-Detection of Coxiella burnetii in Ticks Collected from Sheep and Goats in Southeast Iran

Sr Nourollahi Fard et al. Iran J Arthropod Borne Dis. 2011.

Abstract

Background: There is a little data on Coxiella burnetii (Q fever agent) in Iran. Ticks may play a significant role in the transmission of C. burnetii among animals. The aim of this study was to use polymerase chain reaction for the detection of C. burnetii in ticks collected in Southeast Iran.

Methods: One hundred and sixty ticks were collected from domestic animals in three localities of Kerman Province, Southeast Iran from November to June 2009. The collected ticks were divided into 35 pools and examined by Trans-PCR for C. burnetii.

Results: Three pools, each consisting of five female of Hyalomma anatolicum anatolicum and one pool (6 ticks) of Rhipicephalus sanguineus ticks collected from goats and sheep were found to be positive by Trans-PCR.

Conclusion: This paper documents the first molecular detection of C. burnetii in ticks, which shows their role as putative vectors and reservoirs for this pathogenic agent.

Keywords: Coxiella burnetii; Iran; Ticks; Trans-PCR.

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Figures

Fig. 1.
Fig. 1.
Lanes 1–3, an example of positive samples, lane 4, DNA template from the standard C. burnetii Nine Mile, lane 5, 100-bp DNA ladder, and lane 6, Non Template Control (NTC)
See this image and copyright information in PMC

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