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. 2012 Jul 18:12:109.
doi: 10.1186/1471-2229-12-109.

Improved efficiency of doubled haploid generation in hexaploid triticale by in vitro chromosome doubling

Tobias Würschum  1 , Matthew R Tucker, Jochen C Reif, Hans Peter Maurer
Affiliations

Improved efficiency of doubled haploid generation in hexaploid triticale by in vitro chromosome doubling

Tobias Würschum et al. BMC Plant Biol. .

Abstract

Background: Doubled haploid production is a key technology in triticale research and breeding. A critical component of this method depends on chromosome doubling, which is traditionally achieved by in vivo treatment of seedlings with colchicine.

Results: In this study we investigated the applicability of an in vitro approach for chromosome doubling based on microspore culture. Our results show a pronounced increase in the proportion of doubled haploid triticale plants compared to the spontaneous doubling rate, but also compared to the doubling obtained by the standard in vivo approach. In addition, the frequency of plants surviving from culture medium to maturity is also much higher for the in vitro approach. Colchicine concentrations of 1 mM for 24 h or 0.3 mM applied for 48 or 72 h during the first hours of microspore culture performed best.

Conclusions: Our results suggest that for triticale, in vitro chromosome doubling is a promising alternative to the in vivo approach.

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Figures

Figure 1
Figure 1
Effect of colchicine on embryo formation. Effect of different colchicine concentrations applied for 72 h on embryo formation 4 weeks after microspore isolation.
Figure 2
Figure 2
In vitrochromosome doubling.(A) Heatplot for the rate of fertile doubled haploid plants among regenerated green plants obtained by the tested in vitro methods and by the conventional in vivo approach. (B) Mosaic plot visualizing the contingency table. The area of each tile is proportional to the corresponding cell entry and the colors reflect the residuals (fit or lack of fit) of the loglinear model.
See this image and copyright information in PMC

References

    1. Wedzony M, Forster BP, Zur I, Golemice E, Szechynska-Hebda, Dubas E, Gotebiowska G. In: Progress in doubled haploid technology in higher plants. Touraev A, Forster BP, Mohan Jain S, editor. Advances in Haploid Production in Higher Plants, Springer; 2009. pp. 1–33.
    1. Alheit KV, Reif JC, Maurer HP, Hahn V, Weissmann EA, Miedaner T, Würschum T. Detection of segregation distortion loci in triticale (x Triticosecale Wittmack) based on a high-density DArT marker consensus genetic linkage map. BMC Genomics. 2011;12:380. doi: 10.1186/1471-2164-12-380. - DOI - PMC - PubMed
    1. Reif JC, Maurer HP, Korzun V, Ebmeyer E, Miedaner T, Würschum T. Mapping QTLs with main and epistatic effects underlying grain yield and heading time in soft winter wheat. Theor Appl Genet. 2011;123:283–292. doi: 10.1007/s00122-011-1583-y. - DOI - PubMed
    1. Würschum T. Mapping QTL for agronomic traits in breeding populations. Theor Appl Genet. 2012. DOI 10.1007/s00122-012-1887-6. - PubMed
    1. Maluszynski M, Kasha KJ, Szarejko I. In: Doubled haploid production in crop plants, a manual. Maluszynski M, Kasha KJ, Forster BP, Szarejko I, editor. Kluwer Academic Publishers, Dordrecht, The Netherlands; 2003. Published doubled haploid protocols in plant species; pp. 309–335.

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