Purification of transketolase from baker's yeast by an immunoadsorbent
Affiliations
- PMID: 2282080
Item in Clipboard
Purification of transketolase from baker's yeast by an immunoadsorbent
Biochem Int.
1990 Oct.
Abstract
Baker's yeast transketolase has been purified by immunoaffinity chromatography on specific TK antibodies covalently linked to CNBr-agarose. Affinity chromatography allows a 480-fold purification of TK from yeast extracts and about 80% recovery of the original activity. The isolated enzyme has a specific activity of 12-60 U/mg and during polyacrylamide gel electrophoresis performed at pH 8.9 migrates as two protein bands possessing a transketolase activity which corresponds to two isoforms of the enzyme.
Similar articles
-
[A new method of isolation and a new form of transketolase from baker's yeast].Biokhimiia. 1991 Jun;56(6):1123-30. Biokhimiia. 1991. PMID: 1718447 Russian.
-
A new form of baker's yeast transketolase. An enzyme-RNA complex.FEBS Lett. 1990 Nov 12;274(1-2):27-9. doi: 10.1016/0014-5793(90)81321-e. FEBS Lett. 1990. PMID: 2253777
-
Immunoaffinity purification of rat liver transketolase: evidence for multiple forms of the enzyme.Arch Biochem Biophys. 1986 Feb 15;245(1):212-9. doi: 10.1016/0003-9861(86)90207-9. Arch Biochem Biophys. 1986. PMID: 3947099
-
[Molecular weight and tertiary structure of transketolase from baker's yeast].Biokhimiia. 1978 Mar;43(3):545-54. Biokhimiia. 1978. PMID: 26423 Russian.
-
Purification and properties of glyoxylate reductase I from baker's yeast.J Biochem. 1979 Jul;86(1):105-10. J Biochem. 1979. PMID: 383706
MeSH terms
Substances
LinkOut - more resources
Molecular Biology Databases