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. 1990 Oct;22(1):31-6.

Purification of transketolase from baker's yeast by an immunoadsorbent

N K Tikhomirova  1 , G A Kochetov
Affiliations
  • PMID: 2282080

Purification of transketolase from baker's yeast by an immunoadsorbent

N K Tikhomirova et al. Biochem Int. 1990 Oct.

Abstract

Baker's yeast transketolase has been purified by immunoaffinity chromatography on specific TK antibodies covalently linked to CNBr-agarose. Affinity chromatography allows a 480-fold purification of TK from yeast extracts and about 80% recovery of the original activity. The isolated enzyme has a specific activity of 12-60 U/mg and during polyacrylamide gel electrophoresis performed at pH 8.9 migrates as two protein bands possessing a transketolase activity which corresponds to two isoforms of the enzyme.

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