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. 2013 Jan;18(1):25-33.
doi: 10.1007/s12192-012-0353-3. Epub 2012 Jul 23.

Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats

Felipe Hiroshi Saito  1 , Débora Cristina Damasceno, Bruna Dallaqua, Iara Moreno Linhares, Marilza Vieira Cunha Rudge, Iracema De Mattos Paranhos Calderon, Steven S Witkin
Affiliations

Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats

Felipe Hiroshi Saito et al. Cell Stress Chaperones. 2013 Jan.

Abstract

We evaluated associations between the concentrations of heat shock proteins (hsp60 and hsp70) and their respective antibodies, alterations in maternal reproductive performance, and fetal malformations in pregnant rats with hyperglycemia. Mild diabetes (MD) or severe diabetes (SD) was induced in Sprague-Dawley rats prior to mating; non-treated non-diabetic rats (ND) served as controls. On day 21 of pregnancy, maternal blood was analyzed for hsp60 and hsp70 and their antibodies; and fetuses were weighed and analyzed for congenital malformations. Hsp and anti-hsp levels were correlated with blood glucose levels during gestation. There was a positive correlation between hsp60 and hsp70 levels and the total number of malformations (R = 0.5908, P = 0.0024; R = 0.4877, P = 0.0134, respectively) and the number of malformations per fetus (R = 0.6103, P = 0.0015; R = 0.4875, P = 0.0134, respectively). The anti-hsp60 IgG concentration was correlated with the number of malformations per fetus (R = 0.3887, P = 0.0451) and the anti-hsp70 IgG level correlated with the total number of malformations (R = 0.3999, P = 0.0387). Moreover, both hsp and anti-hsp antibodies showed negative correlations with fetal weight. The results suggest that there is a relationship between hsp60 and hsp70 levels and their respective antibodies and alterations in maternal reproductive performance and impaired fetal development and growth in pregnancies associated with diabetes.

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Figures

Fig. 1
Fig. 1
Fetal weight classification. Fetal weight classification is defined as small for pregnancy age (SPA), appropriate for pregnancy age (APA) or large for pregnancy age (LPA)
Fig. 2
Fig. 2
Concentration of hsp60, hsp70, anti-hsp60, and anti-hsp70 in serum from non-diabetic, mild diabetic, and severe diabetic rats. Sera from rats without diabetes (ND), mild diabetes (MD), and severe diabetes (SD) were tested for hsp60 (a), anti-hsp60 (b), hsp70 (c), and anti-hsp70 (d) by ELISA. Data are presented as mean ± standard deviation. *P < 0.05, compared to ND; #P < 0.05, compared to MD
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