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. 1979 Nov 24;7(6):1485-95.
doi: 10.1093/nar/7.6.1485.

Specific chemical labeling of DNA fragments

H Eshaghpour, D Söll, D M Crothers
Free PMC article

Specific chemical labeling of DNA fragments

H Eshaghpour et al. Nucleic Acids Res. .
Free PMC article

Abstract

We describe a simple method for specific chemical labeling of DNA fragments at their 3'-termini. The procedure includes enzymatic addition of 4-thiouridine, followed by reaction in mild non-denaturing conditions with the highly reactive alpha-haloacetamido derivatives of several chemical labels. The attached reporter molecule can be removed by extended treatment with beta-mercaptoethanol. Among the potential applications of this labeling method is the study of specific protein-DNA interactions in solution.

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