Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Oct;76(10):5090-4.
doi: 10.1073/pnas.76.10.5090.

Interaction site of Escherichia coli cyclic AMP receptor protein on DNA of galactose operon promoters

T Taniguchi, M O'Neill, B de Crombrugghe

Interaction site of Escherichia coli cyclic AMP receptor protein on DNA of galactose operon promoters

T Taniguchi et al. Proc Natl Acad Sci U S A. 1979 Oct.

Abstract

Cyclic AMP (cAMP) and its receptor protein (CRP) have a dual role in the regulation of the two promoters that control the galactose (gal) operon of Escherichia coli. One promoter, P1, requires cAMP-CRP for activity; the other, P2, is inhibited by these factors. We have examined the interactions site of cAMP-CRP on gal DNA by using two types of protection experiments, involving DNase digestion and methylation by dimethyl sulfate. Our results indicate that cAMP-CRP binds to gal DNA in a segment located between 50 and 24 base pairs preceding the P1 start point for transcription. Although the location of the cAMP-CRP interaction site is clearly different in gal and lac DNA, comparison of the DNA sequences suggests a similar recognition sequence. The location of the cAMP . CRP-binding site in gal further suggests that protein-protein interactions between RNA polymerase and cAMP . CRP play an important role in transcription initiation at the gal and possibly other cAMP-dependent promoters.

PubMed Disclaimer

References

    1. J Mol Biol. 1978 Nov 15;125(4):467-90 - PubMed
    1. Nature. 1979 Jun 7;279(5713):494-500 - PubMed
    1. Nature. 1978 Aug 24;274(5673):762-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5314-8 - PubMed
    1. Nucleic Acids Res. 1978 Sep;5(9):3157-70 - PubMed