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. 1979 Sep;76(9):4308-12.
doi: 10.1073/pnas.76.9.4308.

A general priming system employing only dnaB protein and primase for DNA replication

K Arai, A Kornberg

A general priming system employing only dnaB protein and primase for DNA replication

K Arai et al. Proc Natl Acad Sci U S A. 1979 Sep.

Abstract

Priming of phage phi X174 DNA synthesis is effected simply by dnaB protein and primase when the DNA is not coated by single-strand binding protein (SSB). The five prepriming proteins (n,n',n'',i, and dnaC protein) required for priming a SSB-coated phi X174 DNA circle are dispensable. The dnaB protein-primase priming system is also active on uncoated phage G4 and M13 DNAs and on poly(dT). Multiple RNA primers, 10--60 nucleotides long, are transcribed with patterns distinctive for each DNA template. Formation of a stable dnaB protein.DNA complex in the presence of primase and ATP supports the hypothesis that dnaB protein provides a mobile replication promoter signal for primase.

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References

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