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. 2012 Oct;50(10):3378-80.
doi: 10.1128/JCM.01612-12. Epub 2012 Jul 25.

Quantitative genomic and antigenomic enterovirus RNA detection in explanted heart tissue samples from patients with end-stage idiopathic dilated cardiomyopathy

Nicolas Lévêque  1 , Fanny Renois, Déborah Talmud, Yohan Nguyen, François Lesaffre, Camille Boulagnon, Patrick Bruneval, Paul Fornes, Laurent Andréoletti
Affiliations

Quantitative genomic and antigenomic enterovirus RNA detection in explanted heart tissue samples from patients with end-stage idiopathic dilated cardiomyopathy

Nicolas Lévêque et al. J Clin Microbiol. 2012 Oct.

Abstract

Standardized one-step real-time RT-PCR assay detected enterovirus RNA in cardiac biopsy samples from 4 of 20 patients suffering from idiopathic dilated cardiomyopathy (IDCM). The median viral load was 287 copies per microgram of total extracted nucleic acids, with positive- to negative-strand RNA ratios ranging from 2 to 20. These results demonstrate enterovirus persistence in the heart of IDCM patients, characterized by low viral loads and low positive- to negative-RNA ratios.

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Figures

Fig 1
Fig 1
Levels of total viral RNA and negative-strand viral RNA and positive- to negative-strand viral RNA ratios during the first 24 h of CVB3 replication in MA-104 cells.
See this image and copyright information in PMC

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