Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Jul 27;337(6093):421-2.
doi: 10.1126/science.1225787.

Molecular biology. Use and abuse of RNAi to study mammalian gene function

William G Kaelin Jr  1
Affiliations

Molecular biology. Use and abuse of RNAi to study mammalian gene function

William G Kaelin Jr. Science. .
No abstract available

PubMed Disclaimer

Figures

Figure
Figure. si/shRNA screen variables
For a phenotypic si/shRNA screen involving n genes, the ranking of any one gene will be influenced by the knockdown efficiencies of the si/shRNAs (A to D in the example shown) for that gene, their off-target effects (for simplicity, on-target effects not shown), and the dose-response curves linking the activity of that gene and the phenotype being measured (for example, loss of viability).
See this image and copyright information in PMC

References

    1. Elbashir SM, et al. Nature. 2001;411:494. - PubMed
    1. Brummelkamp TR, et al. Science. 2002;296:550. - PubMed
    1. Sui G, et al. Proc. Natl. Acad. Sci. U.S.A. 2002;99:5515. - PMC - PubMed
    1. Prinz F, Schlange T, Asadullah K. Nat. Rev. Drug Discov. 2011;10:712. - PubMed
    1. Begley CG, Ellis LM. Nature. 2012;483:531. - PubMed

Substances