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. 2012 Jul;53(4):516-25.
doi: 10.1093/jrr/rrs002. Epub 2012 Jun 6.

MicroRNA-148b enhances the radiosensitivity of non-Hodgkin's Lymphoma cells by promoting radiation-induced apoptosis

Yong Wu  1 , Guo-Long Liu, Si-Hong Liu, Cai-Xia Wang, Yan-Li Xu, Yi Ying, Ping Mao
Affiliations

MicroRNA-148b enhances the radiosensitivity of non-Hodgkin's Lymphoma cells by promoting radiation-induced apoptosis

Yong Wu et al. J Radiat Res. 2012 Jul.

Abstract

Growing evidence has demonstrated that microRNAs (miRNAs) play an important role in regulating cellular radiosensitivity. This study aimed to explore the role of miRNAs in non-Hodgkin's lymphoma (NHL) radiosensitivity. Microarray was employed to compare the miRNA expression profiles in B cell lymphoma cell line Raji before and after a 2-Gy dose of radiation. A total of 20 differentially expressed miRNAs were identified including 10 up-regulated and 10 down-regulated (defined as P < 0.05). Among the differentially expressed miRNAs, miR-148b was up-regulated 1.53-fold in response to radiation treatment. A quantitative real-time polymerase chain reaction (PCR) assay confirmed the up-regulation of miR-148b after radiation. Transient transfection experiments showed that miR-148b was up-regulated by miR-148b mimic and down-regulated by miR-148b inhibitor in the Raji cells. A proliferation assay showed that miR-148b could inhibit the proliferation of Raji cells before and after radiation. A clonogenic assay demonstrated that miR-148b sensitized Raji cells to radiotherapy. MiR-148b did not affect the cell cycle profile of post-radiation Raji cells compared with controls. An apoptosis assay showed that miR-148b enhanced apoptosis of Raji cells after irradiation. Taken together, these results demonstrate that miR-148b increased the radiosensitivity of NHL cells probably by promoting radiation-induced apoptosis, which suggests that miR-148b plays an important role in the response of NHL to ionizing radiation.

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Figures

Fig. 1.
Fig. 1.
Differentially expressed miRNAs in Raji cells after exposure to 2-Gy X-ray compared with unirradiated control cells (P < 0.05)
Fig. 2.
Fig. 2.
qRT-PCR analysis of miR-148b level in Raji and RL cells. RNA was extracted from Raji and RL cells subjected to different treatments as indicated and analyzed by qRT-PCR
Fig. 3.
Fig. 3.
Altered miR-148b expression after transfection with miR-148b mimic or inhibitor in Raji cells.
Fig. 4.
Fig. 4.
MiR-148b modulated the proliferation of Raji cells after radiation.
Fig. 5.
Fig. 5.
MiR-148b modulated the tumorigenicity of Raji cells after radiation.
Fig. 6.
Fig. 6.
miR-148b had no effects on the cell cycle profiles of Raji cells.
Fig. 7.
Fig. 7.
Overexpression of miR-148b promoted the apoptosis of Raji cells induced by irradiation.
See this image and copyright information in PMC

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