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. 2012 Aug 8;53(32):4202-4205.
doi: 10.1016/j.tetlet.2012.05.156. Epub 2012 Jun 8.

Secondary metabolites produced by fungi derived from a microbial mat encountered in an iron-rich natural spring

Alexandra L Gerea  1 , Katie M Branscum, Jarrod B King, Jianlan You, Douglas R Powell, Andrew N Miller, John R Spear, Robert H Cichewicz
Affiliations

Secondary metabolites produced by fungi derived from a microbial mat encountered in an iron-rich natural spring

Alexandra L Gerea et al. Tetrahedron Lett. .

Abstract

A collection of fungal isolates was obtained from a complex microbial mat, which occupied an iron-rich freshwater spring that feeds into Clear Creek, Golden, Colorado, USA. Two of the fungal isolates, a Glomeromycete (possible Entrophospora sp.) and a Dothideomycete (possible Phaeosphaeria sp.), were investigated for bioactive secondary metabolites. In total, six new compounds consisting of clearanols A-E (5, 6, 10-12) and disulochrin (7) were purified and their structures were determined. Disulochrin exhibited modest antibacterial activity against methicillin-resistant Staphylococcus aureus, whereas clearanol C showed weak inhibitory activity against Candida albicans biofilm formation.

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Figures

Figure 1
Figure 1
Structures of clearanols A–E (5, 6, 10–12) and disulochrin (7).
Figure 2
Figure 2
Key –JH-C 1H-13C HMBC correlations used to determine the structures of 5, 6, 10–12.
Figure 3
Figure 3
Structure of 5 determined by single crystal X-ray diffraction experiment.
See this image and copyright information in PMC

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