Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Jul;6(3):321-7.
doi: 10.5009/gnl.2012.6.3.321. Epub 2012 May 22.

The COX-2-1195AA Genotype Is Associated with Diffuse-Type Gastric Cancer in Korea

Woon Geon Shin  1 , Ha Jung Kim, Sung Jin Cho, Hyoung Su Kim, Kyung Ho Kim, Myoung Kuk Jang, Jin Heon Lee, Hak Yang Kim
Affiliations

The COX-2-1195AA Genotype Is Associated with Diffuse-Type Gastric Cancer in Korea

Woon Geon Shin et al. Gut Liver. 2012 Jul.

Abstract

Background/aims: The potential role of the cyclooxygenase (COX)-2 polymorphism has been reported in relation to the risk of gastrointestinal tract malignancies. Therefore, we investigated whether COX-2 polymorphisms are associated with the risk of gastric cancer (GC) in Korea, one of the areas with a high prevalence of this condition.

Methods: We evaluated the genotypic frequencies of COX-2-765 and -1195 in 100 peptic ulcer patients, 100 GC patients, and 100 healthy controls. The polymorphisms of the COX-2-765 and -1195 genes were analyzed by polymerase chain reaction and restriction fragment length polymorphisms.

Results: The frequencies of the COX-2-1195 GG, GA, and AA genotype were 20%, 60%, and 20% in intestinal-type GC and 8%, 48%, and 44% in diffuse-type GC, respectively (p=0.021). There were no significant differences in the frequency of COX-2-765 genotypes between intestinal-type GC and diffuse-type GC (p=0.603). Age- and sex-adjusted logistic regression analysis showed that the COX-2-1195 AA genotype was the independent risk factor of diffuse-type GC compared with the COX-2-1195 GG genotype (p=0.041; odds ratio, 6.22; 95% confidence interval, 1.077 to 35.870).

Conclusions: The COX-2-1195 AA genotype may render subjects more susceptible to diffuse-type GC.

Keywords: COX-2; Diffuse type; Polymorphism; Stomach neoplasms.

PubMed Disclaimer

Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1
Fig. 1
Restriction patterns for cyclooxygenase (COX)-2-765 with AciI and COX-2-1195 with PvuII. (A) The genotypes of COX-2-765 were designated as follows: GG, 2 bands of 100/210 bp; GC, 3 bands of 100, 210, and 310 bp; and CC, a single band of 310 bp. A 100-bp ladder is displayed in Lane 1 (Marker). Lanes 2, 3, 4, 5, and 6 are GC, GG, GC, GG, and GG, respectively. (B) The genotypes of COX-2-1195 were designated as follows: GG, 2 bands of 50/220 bp; GA, 3 bands of 50, 220, and 270 bp; and AA, a single band of 270 bp. A 100-bp ladder is displayed in Lane 1 (Marker). Lanes 2, 3, and 4 are AA, GG, and GA, respectively.
Fig. 2
Fig. 2
Sequences of the cyclooxygenase-2 promoter region. (A) The single nucleotide polymorphism (SNP) located on nucleotide -765 is represented by arrows. (B) The SNP located on nucleotide -1195 is represented by arrows.
See this image and copyright information in PMC

References

    1. Uemura N, Okamoto S, Yamamoto S, et al. Helicobacter pylori infection and the development of gastric cancer. N Engl J Med. 2001;345:784–789. - PubMed
    1. International Agency for Research on Cancer; WHO/IARC Expert Committee on Cancer Statistics; IARC, editors; WHO/IARC Expert Committee on Cancer Statistics, editors. IARC monographs on the evaluation of carcinogenic risks to humans: schistosomes, liver flukes and Helicobacter pylori. Volume 61. Lyon: IARC; 1994. Infection with Helicobacter pylori; pp. 177–240.
    1. El-Omar EM, Carrington M, Chow WH, et al. Interleukin-1 polymorphisms associated with increased risk of gastric cancer. Nature. 2000;404:398–402. - PubMed
    1. El-Omar EM, Rabkin CS, Gammon MD, et al. Increased risk of noncardia gastric cancer associated with proinflammatory cytokine gene polymorphisms. Gastroenterology. 2003;124:1193–1201. - PubMed
    1. Machado JC, Figueiredo C, Canedo P, et al. A proinflammatory genetic profile increases the risk for chronic atrophic gastritis and gastric carcinoma. Gastroenterology. 2003;125:364–371. - PubMed