doi: 10.1016/0003-2697(90)90140-5.
Assay of aspartylglycosylaminase by high-performance liquid chromatography
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- PMID: 2285152
- DOI: 10.1016/0003-2697(90)90140-5
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Assay of aspartylglycosylaminase by high-performance liquid chromatography
Anal Biochem.
1990 Oct.
Abstract
An aspartylglycosylaminase assay based on high-performance liquid chromatographic analysis of the substrate aspartylglucosamine and product aspartate is described. Aspartylglucosamine and aspartate are derivatized with phenylisothiocyanate and resolved by reverse-phase chromatography. The detection limit for the compounds is 2 pmol. The method can be used for analysis of aspartylglycosylaminase activity in crude cell extracts and tissue samples.
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