Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Aug;87(2):349-58.
doi: 10.4269/ajtmh.2012.11-0666.

Molecular characterization and phylogenetic analysis of dengue viruses imported into Taiwan during 2008-2010

Jyh-Hsiung Huang  1 , Chien-Ling Su, Cheng-Fen Yang, Tsai-Ling Liao, Tung-Chien Hsu, Shu-Fen Chang, Chien-Chou Lin, Pei-Yun Shu
Affiliations

Molecular characterization and phylogenetic analysis of dengue viruses imported into Taiwan during 2008-2010

Jyh-Hsiung Huang et al. Am J Trop Med Hyg. 2012 Aug.

Abstract

We present our surveillance results on imported dengue cases in Taiwan during 2008-2010. A total of 734 imported dengue patients were identified. The travelers were arriving from 18 countries, including Southeast Asia, the Indian subcontinent, South Pacific islands, and Latin America. Gene sequences from 358 dengue virus (DENV) isolates were used to perform phylogenetic analyses, thus, providing an updated view of the geographic distribution and dynamic transmission of DENV strains circulating in these countries. Our results suggest that the DENV-1 genotype I and DENV-2 Cosmopolitan genotype comprise the predominant DENV strains circulating in Southeast Asian countries. The DENV-3 Genotype III strain was found to be newly emerging in several Southeast Asian countries, however, the Asian genotype 2 and the Asian/American genotype of DENV-2 strains appeared to be less prevalent in Southeast Asia. Furthermore, imported dengue viruses are representative of the overall patterns of serotype/genotype frequencies of dengue outbreaks that occurred in Taiwan.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
A phylogenetic tree of dengue virus type 1 (DENV-1). The phylogenetic tree is based on the complete E-gene sequences of 85 strains of DENV-1 including 57 Taiwan isolates from imported and indigenous dengue cases during 2008–2010. The tree was constructed by the maximum likelihood method and the general time-reversible model. Bootstrap support values greater than 75 are shown. The Taiwan isolates from imported cases during 2008–2010 are designated in boldface. The Taiwan isolates from indigenous cases are designated in boldface and solid triangle (▲). Viruses were identified by using the nomenclature of serotype/country/strain/year of isolation/GenBank accession number. The scale bar on the left indicates substitutions per site.
Figure 2.
Figure 2.
A phylogenetic tree of dengue virus type 2 (DENV-2). The phylogenetic tree is based on the complete E-gene sequences of 75 strains of DENV-2 including 54 Taiwan isolates from imported and indigenous dengue cases during 2008–2010. See the legend of Figure 1 for other details.
Figure 3.
Figure 3.
A phylogenetic tree of dengue virus type 3 (DENV-3). The phylogenetic tree is based on the complete E-gene sequences of 63 strains of DENV-3 including 38 Taiwan isolates from imported and indigenous dengue cases during 2008–2010. See the legend of Figure 1 for other details.
Figure 4.
Figure 4.
A phylogenetic tree of dengue virus type 4 (DENV-4). The phylogenetic tree is based on the complete E-gene sequences of 44 strains of DENV-4 including 23 Taiwan isolates from imported and indigenous dengue cases during 2008–2010. See the legend of Figure 1 for other details.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. WHO/TDR . Dengue: guidelines for diagnosis, treatment, prevention and control. New edition. Geneva: WHO; 2009. http://apps.who.int/tdr/svc/publications/training-guideline-publications... Available at. Accessed August 1, 2011. - PubMed
    1. Calisher CH, Karabatsos N, Dalrymple JM, Shope RE, Porterfield JS, Westaway EG, Brandt WE. Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera. J Gen Virol. 1989;70:37–43. - PubMed
    1. Lindenbach BD, Rice CM. Molecular biology of flaviviruses. Adv Virus Res. 2003;59:23–61. - PubMed
    1. Gubler DJ. Dengue and dengue hemorrhagic fever: its history and resurgence as a global public health problem. In: Gubler DJ, Kuno G, editors. Dengue and Dengue Hemorrhagic Fever. New York: CAB International; 1997. pp. 1–22.
    1. Gibbons RV, Vaughn DW. Dengue: an escalating problem. BMJ. 2002;324:1563–1566. - PMC - PubMed

Publication types

Associated data