Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Oct;91(10):927-33.
doi: 10.1177/0022034512456039. Epub 2012 Aug 3.

Microbiological characterization in children with aggressive periodontitis

L M Shaddox  1 , H Huang, T Lin, W Hou, P L Harrison, I Aukhil, C B Walker, V Klepac-Ceraj, B J Paster
Affiliations

Microbiological characterization in children with aggressive periodontitis

L M Shaddox et al. J Dent Res. 2012 Oct.

Abstract

The objective of this study was to characterize the subgingival microbiota of African-American children with Localized Aggressive Periodontitis (LAP). Fifty-one children were included. Subgingival plaque samples were taken from diseased (DD) and healthy sites (DH) in LAP and from healthy sites in HS and HC and analyzed by 16S rRNA-based microarrays. Aggregatibacter actinomycetemcomitans (Aa) was the only species found to be both more prevalent (OR = 8.3, p = 0.0025) and abundant (p < 0.01) in DD. Filifactor alocis (Fa) was also found to be more prevalent in DD (OR 2.31, CI 1.06-5.01, p = 0.03). Most prevalent species in healthy sites were Selenomonas spp, Veillonella spp, Streptococcus spp, Bergeyella sp, and Kingella oralis. Overall, Streptococcus spp, Campylobacter gracilis, Capnocytophaga granulosa, Haemophilus parainfluenzae, and Lautropia mirabilis were most abundant in healthy children, while Aa, Fa, Tannerella sp, Solobacterium moorei, Parvimonas micra, and Capnocytophaga sp were most abundant in LAP. Based on a comprehensive analysis with 16S rRNA-based microarrays, Aa was strongly associated and site-specific in LAP. In contrast, other species were found to be associated with healthy sites and individuals (ClinicalTrials.gov number CT01330719).

Abbreviations: healthy site in healthy sibling (HS); healthy site in healthy control child (HC).

Trial registration: ClinicalTrials.gov NCT01330719.

PubMed Disclaimer

Conflict of interest statement

The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.

Figures

Figure.
Figure.
Bacterial species which showed significantly different prevalence among sites/groups. DD = diseased sites in children with disease; DH = healthy sites in children with disease; HS = healthy sites in healthy sibling; HC = healthy sites in healthy control children. Significant differences denoted by * DD vs. DH, † DD vs. HS, and ‡DD vs. HC.
See this image and copyright information in PMC

References

    1. Alaluusua S, Asikainen S. (1988). Detection and distribution of Actinobacillus actinomycetemcomitans in the primary dentition. J Periodontol 59:504-507 - PubMed
    1. Alfant B, Shaddox LM, Tobler J, Magnusson I, Aukhil I, Walker C. (2008). Matrix metalloproteinase levels in children with aggressive periodontitis. J Periodontol 79:819-826 - PubMed
    1. Armitage GC. (1999). Development of a classification system for periodontal diseases and conditions. Ann Periodontol 4:1-6 - PubMed
    1. Colombo AP, Boches SK, Cotton SL, Goodson JM, Kent R, Haffajee AD, et al. (2009). Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. J Periodontol 80:1421-1432 - PMC - PubMed
    1. Colombo AP, Bennet S, Cotton SL, Goodson JM, Kent R, Haffajee AD, et al. (2012). Impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and “refractory” subjects by the Human Oral Microbe Identification Microarray (HOMIM). J Periodontol [epub ahead of print 2/10/2012] (in press) - PMC - PubMed

Publication types

Associated data