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Review
. 2012 Sep;10(9):599-606.
doi: 10.1038/nrmicro2850. Epub 2012 Aug 6.

High-throughput bacterial genome sequencing: an embarrassment of choice, a world of opportunity

Nicholas J Loman  1 , Chrystala Constantinidou, Jacqueline Z M Chan, Mihail Halachev, Martin Sergeant, Charles W Penn, Esther R Robinson, Mark J Pallen
Affiliations
Review

High-throughput bacterial genome sequencing: an embarrassment of choice, a world of opportunity

Nicholas J Loman et al. Nat Rev Microbiol. 2012 Sep.

Abstract

Here, we take a snapshot of the high-throughput sequencing platforms, together with the relevant analytical tools, that are available to microbiologists in 2012, and evaluate the strengths and weaknesses of these platforms in obtaining bacterial genome sequences. We also scan the horizon of future possibilities, speculating on how the availability of sequencing that is 'too cheap to metre' might change the face of microbiology forever.

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References

    1. Nature. 2010 Mar 11;464(7286):250-5 - PubMed
    1. Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W686-9 - PubMed
    1. Genome Res. 2008 Jul;18(7):1051-63 - PubMed
    1. PLoS One. 2011;6(7):e22751 - PubMed
    1. J Virol. 2009 Jun;83(11):5485-94 - PubMed

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