[Leuconostocs in raw milk: isolation and identification of species rapidly utilizing lactose]

Abstract

Fast lactose fermenting Leuconostoc species and subspecies were isolated from raw milk. Samples were obtained from dairy farms of the surroundings of Buenos Aires city. A lactose, non selective, isolation medium was employed (YCL). Differentiation of leuconostocs from Lactobacillus viridescens and L. confusus was avoided on account of the use of this medium. 801 typical colonies of lactic acid bacteria were selected from YCL agar; 710 of them were identified as lactic acid bacteria from which 114 strains belonged to the genus Leuconostoc. These last strains were then tested for species and subspecies differentiation by dextran production and sugar fermentation. Leuconostoc mesenteroides subsp. dextranicum and L. lactis were identified. Four strains identified as Leuconostoc spp do not belong to any known species.