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. 2012 Nov 5;8(21):3253-6.
doi: 10.1002/smll.201201141. Epub 2012 Aug 8.

Dendronized gold nanoparticles for siRNA delivery

Sung Tae Kim  1 , Apiwat Chompoosor, Yi-Cheun Yeh, Sarit S Agasti, David J Solfiell, Vincent M Rotello
Affiliations

Dendronized gold nanoparticles for siRNA delivery

Sung Tae Kim et al. Small. .
No abstract available

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Figures

Figure 1
Figure 1
Hydrodynamic diameter and zeta potential of G0-AuNP, G1-AuNP, and G2-AuNP. Gel retardation assay of AuNP/β-gal-siRNA complexation at different molar ratios showed a decrease in band intensity due to the fluorescence quenching by complexation with AuNPs.
Figure 2
Figure 2
β-gal gene silencing in SVR-bag4 cell. a) Gene silencing effect of G2-AuNP/β-gal-siRNA with different concentration from 0.021 to 0.084 μM (NP/siRNA=2). b) Gene silencing effect of naked β-gal siRNA, naked nonsense siRNA, G2-AuNP, G2-AuNP/β-gal-siRNA complex at various molar ratios (0.5, 1 and 2), and G2-AuNP/nonsense-siRNA complex (NP/siRNA=2). 0.021μM of siRNA was used in each experiment.
Figure 2
Figure 2
β-gal gene silencing in SVR-bag4 cell. a) Gene silencing effect of G2-AuNP/β-gal-siRNA with different concentration from 0.021 to 0.084 μM (NP/siRNA=2). b) Gene silencing effect of naked β-gal siRNA, naked nonsense siRNA, G2-AuNP, G2-AuNP/β-gal-siRNA complex at various molar ratios (0.5, 1 and 2), and G2-AuNP/nonsense-siRNA complex (NP/siRNA=2). 0.021μM of siRNA was used in each experiment.
Figure 3
Figure 3
Cell viability determined by Alamar blue® assay. Cell viability was determined after the treatment of a) G2-AuNP and b) G2-AuNP/β-gal-siRNA complex at various molar ratios (β-gal siRNA=75 pmol).
Scheme 1
Scheme 1
a) Chemical structures of G0-AuNP, G1-AuNP, and G2-AuNP. b) Schematic illustration of AuNP/ β-gal-siRNA complexation and transfection into cells.
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References

    1. Hamilton AJ, Baulcombe DC. Science. 1999;286:950–952. - PubMed
    2. Castanotto D, Rossi JJ. Nature. 2009;457:426–433. - PMC - PubMed
    3. Jaronczyk K, Carmichael JB, Hobman TC. Biochem. J. 2005;387:561–571. - PMC - PubMed
    1. Dorsett Y, Tuschl T. Nat. Rev. Drug Discov. 2004;3:318–329. - PubMed
    2. Bumcrot D, Manoharan M, Koteliansky V, Sah DWY. Nat. Chem. Biol. 2006;2:711–719. - PMC - PubMed
    3. Sibley CR, Seow Y, Wood MJA. Mol. Ther. 2010;18:466–476. 2010. - PMC - PubMed
    1. Gao K, Huang L. Mol. Pharm. 2009;6:651–658. - PMC - PubMed
    2. Kirchhoff F. Cell. 2008;134:566–568. - PubMed
    1. Takahashi Y, Nishikawa M, Takakura Y. Adv. Drug. Deliv. Rev. 2009;61:760–766. - PubMed
    1. Reischl D, Zimmer A. Nanomedicine. 2009;5:8–20. - PubMed

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