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. 2012 Nov 5;8(21):3253-6.
doi: 10.1002/smll.201201141. Epub 2012 Aug 8.

Dendronized gold nanoparticles for siRNA delivery

Sung Tae Kim  1 , Apiwat Chompoosor, Yi-Cheun Yeh, Sarit S Agasti, David J Solfiell, Vincent M Rotello
Affiliations

Dendronized gold nanoparticles for siRNA delivery

Sung Tae Kim et al. Small. .
No abstract available

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Figures

Figure 1
Figure 1
Hydrodynamic diameter and zeta potential of G0-AuNP, G1-AuNP, and G2-AuNP. Gel retardation assay of AuNP/β-gal-siRNA complexation at different molar ratios showed a decrease in band intensity due to the fluorescence quenching by complexation with AuNPs.
Figure 2
Figure 2
β-gal gene silencing in SVR-bag4 cell. a) Gene silencing effect of G2-AuNP/β-gal-siRNA with different concentration from 0.021 to 0.084 μM (NP/siRNA=2). b) Gene silencing effect of naked β-gal siRNA, naked nonsense siRNA, G2-AuNP, G2-AuNP/β-gal-siRNA complex at various molar ratios (0.5, 1 and 2), and G2-AuNP/nonsense-siRNA complex (NP/siRNA=2). 0.021μM of siRNA was used in each experiment.
Figure 2
Figure 2
β-gal gene silencing in SVR-bag4 cell. a) Gene silencing effect of G2-AuNP/β-gal-siRNA with different concentration from 0.021 to 0.084 μM (NP/siRNA=2). b) Gene silencing effect of naked β-gal siRNA, naked nonsense siRNA, G2-AuNP, G2-AuNP/β-gal-siRNA complex at various molar ratios (0.5, 1 and 2), and G2-AuNP/nonsense-siRNA complex (NP/siRNA=2). 0.021μM of siRNA was used in each experiment.
Figure 3
Figure 3
Cell viability determined by Alamar blue® assay. Cell viability was determined after the treatment of a) G2-AuNP and b) G2-AuNP/β-gal-siRNA complex at various molar ratios (β-gal siRNA=75 pmol).
Scheme 1
Scheme 1
a) Chemical structures of G0-AuNP, G1-AuNP, and G2-AuNP. b) Schematic illustration of AuNP/ β-gal-siRNA complexation and transfection into cells.
See this image and copyright information in PMC

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