[Protection against Salmonella via immunization with recombinant lactic acid bacteria]

Abstract

Mucosal immunization of hens may be effective to prevent contamination of Salmonella enterica serovar Enteritidis (SE) in eggs. Lactic acid bacteria have been considered potential vaccine delivery agents because they are safe, immunogenic, and inexpensive. Our research group has been investigating the development of oral vaccines against SE using a Lactobacillus casei strain as an antigen delivery vehicle. Recombinant lactobacilli expressing SE antigens FliC and SipC have been constructed and administered to mice. Antigen specific immune responses and protective immunity were elicited after the immunization. For adjuvant delivery, IL-1beta-secreting L. casei was also engineered and its efficacies were evaluated in vitro and in vivo. This article reviews a novel approach to develop SE vaccines using recombinant lactobacilli.