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. 2012 Nov;50(11):3722-5.
doi: 10.1128/JCM.02038-12. Epub 2012 Aug 15.

Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated ampC β-lactamase genes

Chelsie N Geyer  1 , Mark D Reisbig, Nancy D Hanson
Affiliations

Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated ampC β-lactamase genes

Chelsie N Geyer et al. J Clin Microbiol. 2012 Nov.

Abstract

A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.

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Figures

Fig 1
Fig 1
Evaluation of ampC TaqMan multiplex PCR assay. (A and B) Detection and FAM fluorescence of AmpC transformant controls (A) and HEX fluorescence of the 16S ribosomal internal control (B) for AmpC transformants and non-AmpC-producing clinical strains tested in panel A. (C and D) FAM fluorescence of positive AmpC transformant controls and an unknown clinical isolate from CUMC (C) and HEX fluorescence of the 16S ribosomal internal control for those strains tested in panel C (D). Arrows indicate the specific ampC transformant control or unknown clinical isolate detected; circles indicate the group of strains tested or no template controls.
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