Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Mar;7(2):99-106.
doi: 10.1007/BF00203820.

Three-dimensional (1)H-TOCSY-relayed ct-[(13)C, (1)H]-HMQC for aromatic spin system identification in uniformly (13)C-labeled proteins

O Zerbe  1 , T Szyperski, M Ottiger, K Wüthrich
Affiliations

Three-dimensional (1)H-TOCSY-relayed ct-[(13)C, (1)H]-HMQC for aromatic spin system identification in uniformly (13)C-labeled proteins

O Zerbe et al. J Biomol NMR. 1996 Mar.

Abstract

Three-dimensional (1)H-TOCSY-relayed ct-[(13)C,(1)H]-HMQC is a novel experiment for aromatic spin system identification in uniformly (13)C-labeled proteins, which is implemented so that it correlates the chemical shift of a given aromatic proton with those of the directly attached carbon and all vicinal protons. The ct-HMQC scheme is used both for overlay of the indirect (1)H and (13)C chemical shift evolution periods and for the generation of (1)H-(1)H antiphase magnetization to accelerate the (1)H-TOCSY magnetization transfer at short mixing times. As an illustration, data recorded for the 18 kDa protein cyclophilin A are presented. Since transverse relaxation of (13)C-(1)H zero-quantum and double-quantum coherences is to first order insensitive to (13)C-(1)H heteronuclear dipolar relaxation, the new experiment should work also for proteins with molecular weights above 20 kDa.

PubMed Disclaimer

References

    1. J Biomol NMR. 1993 Jan;3(1):127-32 - PubMed
    1. J Biomol NMR. 1991 Sep;1(3):299-304 - PubMed
    1. J Biomol NMR. 1995 Nov;6(3):335-9 - PubMed
    1. J Biomol NMR. 1994 Jul;4(4):463-82 - PubMed
    1. Protein Sci. 1994 Oct;3(10):1806-21 - PubMed