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Comment
. 2012 Sep 4;109(36):14289-90.
doi: 10.1073/pnas.1212246109. Epub 2012 Aug 21.

Sequence error storms and the landscape of mutations in cancer

Stefan Kirsch  1 , Christoph A Klein
Affiliations
Comment

Sequence error storms and the landscape of mutations in cancer

Stefan Kirsch et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Sequence error correction by different methods. In the example given, the DNA has one somatic mutation (on both strands); both sample preparation and NGS add artifactual mutations as a consequence of deamination events, oxidative damage, and errors typical for the applied sequencing instrument. Standard methods for the correction of sequencing errors use the abundance of a sequence read to identify errors. SSCS and the analogous Safe-SeqS (6) use unique identifiers on single-strand molecules to group families of sequence reads and remove those that occur less frequently than in 95% of family reads. Duplex sequencing, in addition to assigning families of single-strand reads, exploits the information from the complementary strand. First, the complementary strand is identified via the complementary sequence tags on both strands, and only bases are kept that are present on both strands. Thereby, the correct sequence is identified.
See this image and copyright information in PMC

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References

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